Large Glucagon Immunoreactivity in Extracts of Pancreas

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Large glucagon immunoreactivity in extracts of pancreas.

The glucagon immunoreactivity present in canine pancreatic extracts can be separated by gel filtration into two fractions; one fraction, comprising over 90% of the immunoreactivity, is similar in molecular size to the glucagonla11 marker, while the other appears to be at least twice as large. Incubation of this large glucagon immunoreactivity (LGI) in solutions such as 1 M acetic acid, 8 M urea...

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The biosynthesis of glucagon was studied by using the recirculated, isolated perfused rat pancreas. [3H]Tryptophan was initially incorporated into acid-ethanol-extractable protein, which on gel filtration was eluted with a molecular weight ofabout 9000 and contained a small amount of glucagon immunoreactivity. With longer incubation [3H]tryptophan incorporation into a second peak was obtained i...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1970

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)63360-5